Biosynthesis and metabolism of trigonelline in Lemna paucicostata 151.

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Threonine Synthase of Lemna paucicostata Hegelm. 6746.

Threonine synthase (TS) was purified approximately 40-fold from Lemna paucicostata, and some of its properties determined by use of a sensitive and specific assay. During the course of its purification, TS was separated from cystathionine gamma-synthase, establishing the separate identity of these enzymes. Compared to cystathionine gamma-synthase, TS is relatively insensitive to irreversible in...

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Aspartokinase of Lemna paucicostata Hegelm. 6746.

A sensitive and specific method was developed for assay of aspartokinase (EC 2.7.2.4) in crude extracts of Lemna paucicostata. Lysine inhibited approximately 93%, and threonine approximately 6%; together, these amino acids inhibited 99%. Inhibition by lysine was synergistically increased by S-adenosylmethionine, which by itself had no effect on activity. Essentially complete inhibition of threo...

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Homocysteine Biosynthesis in Green Plants: Physiological Importance of the Transsulfuration Pathway in Lemna paucicostata.

To permit an assessment of the relative contributions of the transsulfuration and the direct sulfhydration pathways for homocysteine biosynthesis, the time course of incorporation of (35)S from (35)SO(4) (2-) into various sulfur-containing compounds in Lemna paucicostata has been determined. Plants were grown with either low (4.5 micromolar) or ample (1,000 micromolar) sulfate in the medium. At...

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C14-oxylipin glucosides isolated from Lemna paucicostata.

Oxylipin glucosides (2-4) were isolated from Lemna paucicostata with their structures and absolute configurations elucidated by spectroscopic and chemical methods. Compounds 2-4 were glucosides of C14 oxylipin which were synthesized from alpha-linolenic acid via the 9-lipoxygenase pathway.

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Biosynthesis of trigonelline.

Trigonelline, N-methyl nicotinic acid, was first noted in the seeds of Trigonella joenum-graecum (the fenugreek) (1) and has since been found in the seeds and tubers of many species (2,3) as well as in leaves of one species of pea (4). Although one may assume that trigonelline arises metabolically from nicotinic acid, definitive evidence has been lacking. In the course of several attempts to de...

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ژورنال

عنوان ژورنال: Agricultural and Biological Chemistry

سال: 1989

ISSN: 0002-1369,1881-1280

DOI: 10.1271/bbb1961.53.2867